5368 Tubulin Polymerization in Dimethyl Sulfoxide

The self-assembly of tubulin devoid of microtubuleassociated proteins (MAPs) has been studied using a MES buffer containing dimethyl sulfoxide (MezSO). Between 6% and 12% v/v MeZSO, the tubulin forms polymers which resemble microtubules in their morphology and chemical properties. These MezSO microtubules, like normal microtubules, require GTP for assembly and are sensitive to cold, calcium ions, colchicine, and hydrostatic pressure. The polymerization shows a critical concentration which is dependent on the concentration of MezSO. 8% MezSO was found to be the optimum concentration for microtubule assembly. In these conditions, a linear Van t'Hoff plot is obtained, with AHo/kJ*mol" = 26.5 over the range 10-35 "C, and ASo/J=K"-mol" = 186, in contrast to the assembly with MAPS or glycerol. The kinetics of polymerization shows that the apparent stoichiometry coefficient of nucleation has the value of 2. Ultracentrifugation analysis shows that there are no oligomers present at low temperatures in the absence of free nucleotide, while in identical conditions, tubulin with MAPs does form oligomers. Although the solvent conditions used supported propagation of assembly, nucleation was found to be very dependent on the transiently locally high MezSO concentrations formed when MezSO was added to initiate assembly. It is concluded that Me2SO preferentially stabilizes the lateral interactions.